מחקרים על פריון הגבר (אנגלית)
EFFECT OF DIETARY DHA SUPPLEMENTATION ON SPERM DNA INTEGRITY.
J. C. Martinez-Soto, J. C. Domingo, B. Cordobilla, L. Palbero, A. Pellicer, J. Landeras. IVI Murcia, Murcia, Spain; Biochemistry and Molecular Biology, University of Barcelona, Barcelona, Spain; IVI Valencia, Valencia, Spain.
FERTILITY AND STERILITY
OBJECTIVE: Oxidative stress is an imbalance between the amount of reactive oxygen species (ROS) produced and the ability of the antioxidants to scavenge these .High levels of ROS produces DNA damage which could cause infertility. Docosahexaenoic acid (DHA) is a polyunsaturated Omega-3 fatty acid and is found in high concentrations in sperm membranes. Several studies suggest an antioxidant capacity for DHA. The author assumed that oxidant stress is oxidizing DNA and fragmenting it as one of the major causes to infertility. The aim of this study is to evaluate the effect of AXOM3, enzymatic nutraceutical triglyceride oil with a high concentration of DHA in damaged spermatozoa DNA.
DESIGN: A randomised double-blind study with a control group.
MATERIALS AND METHODS: 46 male patients were included in the present study. DHA group A(n=21) was given a supplement of 1050mg/day AXOM3 for 10 weeks. The placebo group B (n=15) was given a supplement of 1050mg/day of sunflower oil for 10 weeks. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL)staining was used to determine sperm DNA fragmentation.The total antioxidant capacity of seminal plasma was measured using myoglobin, which interacts with 2,2-azinobis(3ethylbenzo-thiazoline6sulphonate)(ABTS).The latency phase (Lag) in the accumulation of ABTS cation is proportional to antioxidant concentration.
RESULTS: In group A, there was a statistically significant decrease in DNA fragmentation which is directly proportional to the number of weeks of treatment (0 weeks 25.98 +_ 4.73, 5 weeks 15.60 +_2.46, 10 weeks 8.79 +_ 1.92) P< 0.01.
In group B, DNA fragmentation was not affected by treatment (0 weeks 17.81 +_ 2.56, 5 weeks 23.50 +_ 4.55,10 weeks 29.01 +_ 6.13) P =0.25.
No significant variations in the level of DHA were observed in the spermatozoa membrane or in the seminal plasma. Lag showed significantly greater values in group A patients vs group B patients.
CONCLUSION: The results show that dietary DHA supplementation decreases sperm DNA damage and improves the antioxidant system. Therefore AXOM3 can be used as a therapy of choice for sperm with high DNA fragmentation.
DHA AND MEN SEMEN FERTILITY.
Docosahexaenoic acid supplementation fully restores fertility and spermatogenesis in male delta-6 desaturase-null mice.
Volume: 51, Issue: 2, Publisher: The American Society for Biochemistry and Molecular Biology, Pages: 360-367
Journal of Lipid Research (2010)
The statistics are showing that in the USA alone 6.1% of individuals suffer from infertility problems, out of which 30-35 % are males.
It is also well documented that DHA is present in the membrane of spermatozoa in very large concentrations.
Scientists deducted from this data that DHA is correlated to fertility and its deficiency is causing infertility.
Yet, only recently Prof Nakamura from the University of Illinois managed to carry out very exciting experiments that caused a big sensation in the academic and nutritional world. His work and collaborators entitled: “Docosahexaenoic acid supplementation fully restores fertility and spermatogenesis in male delta-6 desaturase-null mice” was published in J. Lipid Res. 51, 360, (2010)
His work is cited by many important popular and scientific journals.
Nakamura used genetically modified mice that lack the gene responsible for the enzyme leading to the synthesis of DHA in the mice and human. These mice were found to be infertile and could not breed.
He supplemented them with 0.3 gr per day of DHA or arachidonic acid (AA) for 16 weeks and found surprisingly that the mice fed with AA remained non-fertile, but those fed with DHA restored all reproduction impairments.
Nakamura tested sperm count, sperm shape, sperm mobility and orientation, and also the capability of breeding.
To his surprise all these parameters were completely restored with the supplementation of DHA and the mice could breed again as normal.
The summary of his work was published recently by many journals and were entitled sensational. The mechanism of this activity is yet to be studied.
In summary the benefits of DHA in relation to human fertility are:
- Increase Your Semen Volume.
- Boost Your Sperm Count.
- Engender Youthful Sexual Response.
- Extend Your Sexual Stamina.
- Strengthen Your Ejaculation.
- Intensify Your Orgasms
Enclosed is the abstract of the original research report:
Delta-6 desaturase-null mice (/) are unable to synthesize highly unsaturated fatty acids (HUFAs): arachidonic acid (AA), docosahexaenoic acid (DHA), and n6-docosapentaenoic acid (DPAn6). The males exhibit infertility and arrest of spermatogenesis at late spermiogenesis. To determine which HUFA is essential for spermiogenesis, a diet supplemented with either 0.2% (w/w) AA or DHA was fed to wild-type (+/+) and males at weaning until 16 weeks of age (n = 35). A breeding success rate of DHA-supplemented was comparable to +/+. DHA-fed showed normal sperm counts and spermiogenesis. Dietary AA was less effective in restoring fertility, sperm count, and spermiogenesis than DHA. Testis fatty acid analysis showed restored DHA in DHA-fed /, but DPAn6 remained depleted. In AA-fed /, AA was restored at the level, and 22:4n6, an AA elongated product, accumulated in testis. Cholesta-3,5-diene was present in testis of and DHA-fed /, whereas it diminished in and AA-fed /, suggesting impaired sterol metabolism in these groups. Expression of spermiogenesis marker genes was largely normal in all groups. In conclusion, DHA was capable of restoring all observed impairment in male reproduction, whereas 22:4n6 formed from dietary AA may act as an inferior substitute for DHA.